Magazi Otolera Kupatukana Gel Tube
Kufotokozera Kwachidule:
Amakhala ndi gel apadera omwe amalekanitsa maselo a magazi kuchokera ku seramu, komanso tinthu tating'onoting'ono toyambitsa magazi kuti titseke mwamsanga.Zitsanzo za magazi zimatha kukhala centrifuged, kulola kuti seramu yomveka bwino ichotsedwe kuti iyesedwe.
Pamene seramu yachisanu ikufunika, ikani machubu otengera pulasitiki nthawi yomweyo mufirijiDziwitsani woimira wanu waluso kuti muli ndi chitsanzo chozizira choti musankheup; Zitsanzo zosiyana zowumitsidwa ziyenera kuperekedwa pamayeso aliwonse omwe amafunikira chitsanzo chozizira.Machubu Olekanitsa Serum (SST).Cholekanitsa seramu (Golide, pamwamba pamiyendo yofiira/yotuwira)Machubu amakhala ndi magaziactivator ndi gel osakaniza kuti alekanitse seramu ndi maselo koma osaphatikiza anticoagulant.kugwiritsa ntchito chubu cholekanitsa seramu; Musagwiritse ntchito machubu olekanitsa seramu kuti mupereke zitsanzo zomwe tricyclicMa antidepressants, Direct Coombs', Gulu la Magazi, ndi Mitundu amafunsidwa.
1. Kujambula magazi athunthu mu kuchuluka kwa 21/2 kuchuluka kwa seramu yofunikira kuti mulingo wokwanira waSeramu imatha kupezeka. Chubu chapamwamba cha 5 mL Gold chidzapereka pafupifupi 2 mL seramu pambuyo pakuundana ndicentrifuging.Chubu chapamwamba cha 10mL chokhala ndi matope ofiira/imvi chimatulutsa pafupifupi seramu 4mL. Lembetsani chitsanzocho.moyenera.
2.Pang'onopang'ono mutembenuzire chubu cholekanitsa seramu kasanu kuti musakanize choyambitsa magazi ndi magazi.
3.Ikani chubu chotolera mowongoka pachoyikapo, ndikulola magazi kuti atseke kutentha kwa firiji.kwa mphindi zosapitirira 30-45. (Zovala nthawi zambiri zimapanga mphindi 20-30.)
4.Mutatha kulola kuti magaziwo apange mphindi 20-30, ikani chubu mu centrifuge, stopper end up.centrifuge kwa mphindi 15 pa liwiro lolimbikitsa ndi wopanga.Musalole nthawi yayitalicentrifugation chifukwa izi zingayambitse hemolysis. Mukamagwiritsa ntchito bench-top centrifuge, gwiritsani ntchito chubu choyezera.mtundu womwewo wokhala ndi voliyumu yofanana yamadzi.
5.Zimitsani centrifuge ndikuilola kuti iyime.Osayimitsa ndi dzanja kapena brake.Chotsanichubu mosamala popanda kusokoneza zomwe zili mkati.Yang'anani chotchinga gel osakaniza kuti muwonetsetse kuti chatseka seramu kuchokera.Yang'ananinso mu seramu kuti muwone zizindikiro za hemolysis (mtundu wofiira) ndi turbidity (mkaka kapena opaque)gwirani mpaka kuwala. Onetsetsani kuti mwapereka labotale kuchuluka kwa seramu yomwe yatchulidwa.
6.Make sure thechubu imalembedwa bwino ndi zonse zofunikira kapena bar code.
7.Ngati chitsanzo chozizira sichikufunika, sikoyenera kusamutsa seramu ku chubu choyendera pulasitiki.
8. Pameneseramu yachisanu ikufunika, nthawi zonse tumizani seramu (pogwiritsa ntchito pipette) kukhala yosiyana, yolembedwa bwino.pulasitiki kutumiza chubu Ikani chubu nthawi yomweyo mu mufiriji mu firiji, ndipo dziwitsaniwoimira ntchito zaukatswiri kuti muli ndi chitsanzo chozizira choti mutenge. Musamawuze seramu yagalasiseparator chubu. Tumizani chubu chosinthira cha pulasitiki cholembedwa momveka bwino pamayesero aliwonse ofunikira zitsanzo zowumitsidwa.Pokhapokha zitasonyezedwa mwanjira ina, zitsanzo za seramu zitha kutumizidwa kutentha kozizira.
