Magazi a Chitsanzo Chotolera Gray Tube
Kufotokozera Kwachidule:
Chubuchi chimakhala ndi potaziyamu oxalate monga anticoagulant ndi sodium fluoride monga chosungira - chomwe chimagwiritsidwa ntchito kusunga shuga m'magazi athunthu komanso mayeso apadera a chemistry.
Ngati plasma ikufunika, tsatirani izi.
1.Nthawi zonse gwiritsani ntchito chubu choyezera choyezetsa chomwe chimafuna anticoagulant yapadera (mwachitsanzo, EDTA,heparin,sodium citrate, etc) kapena preservative.
2.Dinani chubu mofatsa kuti mutulutse zowonjezera kumamatira ku chubu kapena diaphragm yoyimitsa.
3.Lolani chubu chofufumitsa kuti chidzaze kwathunthu.Kulephera kudzaza chubu kumayambitsa magazi osayenera.chiŵerengero cha anticoagulant ndikupereka zotsatira zokayikitsa zoyesa.
4.Kupewa kutsekeka, sakanizani magazi ndi anticoagulant kapena preservative mukangojambula chilichonseKuonetsetsa kusakanikirana kokwanira, tembenuzani chubu pang'onopang'ono kasanu mpaka kasanu ndi kamodzi pogwiritsa ntchito kuzungulira kwa dzanja.kuyenda.
5.Mwamsanga centrifuge chitsanzo kwa 5minutes.Musachotse choyimitsa.
6.Zimitsani centrifuge ndikuilola kuti iyime. Osayimitsa ndi dzanja kapena brake. Chotsanichubu mosamala popanda kusokoneza nkhani.
7.Ngati mulibe Light Green pamwamba chubu(Plasma Separator chubu), chotsani choyimitsa ndikulakalaka mosamala.madzi a m'magazi, pogwiritsa ntchito pasteur pipette yotayika pa chubu chilichonse. Ikani nsonga ya pipette pambaliya chubu, pafupifupi 1/4 inchi pamwamba pa cell layer. Osasokoneza wosanjikiza wa cell kapena kunyamula ma cellmu pipette.Osatsanulira; gwiritsani ntchito pipette.
8. Tumizani plasma kuchokera ku pipette kupita ku chubu chosinthira. Onetsetsani kuti mukupereka labotale ndi kuchuluka kwaplasma yotchulidwa.
9. Lembani machubu onse momveka bwino komanso mosamala ndi zonse zofunikira kapena bar code.Machubu onse ayenera kulembedwandi dzina lonse la wodwalayo kapena nambala yake yodziwikiratu momwe ikuwonekera pa fomu yofunsira mayeso kapena khodi ya bar.Komanso, sindikizani pa chizindikiro mtundu wa madzi a m'magazi omwe atumizidwa (mwachitsanzo, "Plasma, Sodium Citrate," "Plasma, EDTA," ndi zina zotero).
10.Pamene madzi a m'magazi owuma akufunika, ikani machubu otengera pulasitiki nthawi yomweyo mufiriji.firiji, ndipo dziwitsani woimilira wanu wantchito kuti muli ndi chitsanzo chozizira chomwe chiyenera kusankhidwapamwamba.
