Single Muclear Cell Gel Separation Tube-CPT Tube

Kufotokozera Kwachidule:

Amagwiritsidwa ntchito kupatula ma monocytes kumagazi athunthu.

Amagwiritsidwa ntchito makamaka pakuzindikira chitetezo chamthupi cha ma lymphocyte monga HLA, kuzindikira kwamtundu wotsalira wa leukemia ndi chithandizo cha chitetezo chamthupi.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kodi CPT Tube Ndi Chiyani

Single muclear cell gel seperation chubu (CPT chubu) imawonjezedwa ndi hypaque, anticoagulant ndi gel olekanitsa.Lymphocytes ndi maselo a mononuclear amatha kukhala olekanitsidwa mosavuta ndi magazi athunthu ndi sitepe imodzi centrifugation pogwiritsa ntchito gel wapadera wolekanitsa maselo.Amagwiritsidwa ntchito kwambiri pozindikira za chitetezo chamthupi cha lymphocyte, HLA kapena kuzindikira kwa jini yotsalira ya leukemia ndi chithandizo cha chitetezo chamthupi.Imakhala njira muyezo kwa sitepe imodzi m'zigawo za monocytes kwa matenda diagnostic chitsanzo kukonzekera ndi ma immunotherapy.

Ntchito Zogulitsa

1) Kukula: 13 * 100mm, 16 * 125mm;

2) Voliyumu yowonjezera: 0.1ml, 135usp;

3) Kuchuluka kwa Magazi: 4ml, 8ml;

4) Shelf Life: Miyezi 24 kuyambira tsiku lopangidwa;

5) Kusungirako:Sungani pa 18-25℃.

ZogulitsaUbwino

1) Yothandiza, yolondola komanso yotetezeka;

2) Ndi Ficoll Hypaque yomangidwa, anticoagulant ndi gel olekanitsa, maselo a mononuclear amasiyanitsidwa ndi magazi athunthu ndi sitepe imodzi centrifugation.

3) Tekinoloje yolondola yolekanitsa ma cell.

4) Inner khoma bionic nembanemba processing luso;

5) Kuchira kwa monocyte kumaposa 90%, chiyero ndi choposa 95%, ndipo kupulumuka kumaposa 99%

Nkhani Zofunika Kusamala

Mfundo zotsatirazi ziyenera kuzindikirika:

1) Pamene mukuchita kuyesera kwa culturing maselo, tcherani khutu ntchito aseptic, samatenthetsa reagents (kupatukana njira, kutsuka njira, etc.) ndi zida.Ntchitoyi iyenera kuchitidwa ndi akatswiri kuti atsimikizire kugwirizana kwa ntchitoyo.

2) Kutentha kwa Centrifugation nthawi zambiri kumakhala kutentha (2 ~ 25 ℃).

3) Kawirikawiri polekanitsa maselo a mononuclear (PBMC) ndi Ficoll, kuchuluka kwa maselo ofiira a magazi ndi ochepa, omwe angapangitse kulondola kwa kuyesa.Choncho lysate angagwiritsidwe ntchito (ena ayenera chosawilitsidwa), kulamulira lysis nthawi ndi kupewa kumakhudza ntchito maselo mononuclear.

4) Samalani ndi re-centrifugation yomwe imatha kuwirikiza kawiri.

Zam'mbuyo: CTAD Detection Tube

Ena: Red Plain Blood Tube