Madzi a m'magazi a Platelet amalimbikitsa angiogenesis mu mbewa zomwe zingalimbikitse kukula kwa tsitsi

Platelet-rich plasma (PRP) ndi plasma yochuluka ya maselo a anthu mu plasma.Kupyolera mu kuchepa kwa ma alpha granules m'mapulateleti, PRP ikhoza kutulutsa zinthu zosiyanasiyana za kukula, kuphatikizapo platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), ndi kusintha. kukula factor (TGF), zomwe zalembedwa kuti ayambe kuchiritsa mabala ndi kulimbikitsa kuchulukana ndi kusintha kwa endothelial maselo ndi pericytes mu endothelial zikumera.

Ntchito za PRP zochizira kukula kwa tsitsi zafotokozedwa m'mafukufuku ambiri aposachedwa.Uebel et al.apeza kuti zinthu za kukula kwa mapulateleti a plasma zimachulukitsa zokolola za ma follicular unit mu opaleshoni yachimuna ya dazi.Ntchito yaposachedwa yawonetsa kuti PRP imachulukitsa kuchuluka kwa ma cell a dermal papilla ndikupanga kusintha mwachangu kwa telogen-to-anagen pogwiritsa ntchito mitundu ya vivo ndi in vitro.Kafukufuku wina wasonyeza kuti PRP imalimbikitsa kukonzanso tsitsi la tsitsi ndikufupikitsa kwambiri nthawi yopangira tsitsi.

Zonse za PRP ndi plasma-poor-platelet (PPP) zikuphatikizapo zonse zowonjezera mapuloteni a coagulation.Mu phunziro lino, chikoka cha PRP ndi PPP pa kukula kwa tsitsi mu C57BL / 6 mbewa zinafufuzidwa.Lingaliro linali lakuti PRP inali ndi zotsatira zabwino pa kukula kwa tsitsi ndi kuwonjezeka kwa chiwerengero cha ma follicles a tsitsi.

Zinyama zoyesera

Konse 50 athanzi C57BL/6 mbewa zazimuna (6 masabata, 20 ± 2 g) zinapezedwa kuchokera ku Center of Laboratory Animals, Hangzhou Normal University (Hangzhou, China).Nyama zinkadyetsedwa chakudya chofanana ndi kusungidwa m’malo osasinthasintha pansi pa 12:12-h light-dark cycle.Pambuyo pa sabata la 1 la acclimatization, mbewa zinagawidwa mwachisawawa m'magulu atatu: gulu la PRP (n = 10), gulu la PPP (n = 10), ndi gulu lolamulira (n = 10).

Ndondomeko yophunzirira idavomerezedwa ndi komiti yoyang'anira zamakhalidwe ofufuza zanyama pansi pa Law of Animal Research and Statutory Regulations ku China.

Kuyeza kutalika kwa tsitsi

Pa 8, 13, ndi masiku 18 pambuyo pa jekeseni womaliza, tsitsi la 10 pa mbewa iliyonse linasankhidwa mwachisawawa m'dera lomwe mukufuna.Kuyeza kutalika kwa tsitsi kunachitika m'magawo atatu pogwiritsa ntchito maikulosikopu ya elekitironi, ndipo avareji yawo idawonetsedwa ngati mamilimita.Tsitsi lalitali kapena lowonongeka silinaphatikizidwe.

Hematoxylin ndi eosin (HE) kudetsa

Zitsanzo za khungu la dorsal zidachotsedwa pamasiku 18 pambuyo pa jekeseni lachitatu.Kenako zitsanzo zidakhazikitsidwa mu 10% osalowerera ndale, ophatikizidwa mu parafini, ndikudula 4 μm.Zigawozo zinaphikidwa kwa 4 h kuti deparaffinization pa 65 ° C, yoviikidwa mu gradient ethanol, ndiyeno yodetsedwa ndi hematoxylin kwa 5 min.Pambuyo posiyanitsidwa ndi 1% hydrochloric acid mowa, zigawozo zidakulungidwa m'madzi ammonia, odetsedwa ndi eosin, ndikutsukidwa ndi madzi osungunuka.Pomaliza, zigawozo zidathiridwa madzi ndi ethanol ya gradient, yotsukidwa ndi xylene, yoyikidwa ndi utomoni wosalowerera, ndipo idawonedwa pogwiritsa ntchito ma microscope (Olympus, Tokyo, Japan).