Gel Yellow Blood Collection Tube

Machubu otolera magazi opatukana a gel akhala akugwiritsidwa ntchito kwambiri m'ma laboratories azachipatala.Kusiyanitsa gel osakaniza kumatha kupanga kusanjikiza kwapadera pakati pa zigawo za cell ndi seramu (plasma), kuteteza bwino kusinthana kwa zinthu pakati pa maselo a magazi ndi seramu (plasma), ndikuwonetsetsa kukhazikika kwa zigawo za seramu (plasma) mkati mwa nthawi inayake.Kulekanitsa guluu makamaka wapangidwa silikoni mphira, macromolecular hydrocarbons, guluu hydrophobic, etc. Monga polima zakuthupi, ndi insoluble m'madzi ndi inert.Ndi madzi a thixotropic viscous omwe ali ndi mphamvu ya 1.04-1.05 mmol / Pakati pa L, ali ndi ubwino wa kukana kwa okosijeni, kukana kutentha kwambiri, kutsika kwa kutentha, komanso kutsekemera kwa mpweya wabwino.Kuchulukana kwa seramu ndi 1.026-1.031 mmol / L, ndipo hematocrit ndi 1.090-1.095.Chifukwa cha mphamvu yokoka yeniyeni, gel olekanitsa ali pakati pa seramu ndi maselo a magazi, choncho nthawi zonse, magazi adzawoneka motsatizana pambuyo pa centrifugation.Seramu, gel olekanitsa, ndi maselo a magazi 3 pansi.

Pali mitundu iwiri ya machubu olekanitsa magazi a gel omwe amagwiritsidwa ntchito m'ma labotale: chubu cholekanitsa cha seramu gel procoagulation chubu ndi plasma kupatukana gel anticoagulation chubu.Seramu kupatukana gel procoagulation chubu ndi kuwonjezera coagulant mu chubu chosonkhanitsa magazi kufupikitsa nthawi coagulation magazi, kupeza seramu mofulumira, ndi kunena zotsatira mu nthawi yochepa.Machubu osonkhanitsira magazi agalasi safunikira kuwonjezera ma coagulant, ndipo magazi olumikizana ndi khoma la chubu lagalasi amayambitsa kukomoka.Komabe, zinthu za coagulation XI ndi XII zikakumana ndi machubu osonkhanitsira magazi apulasitiki, kuthekera kwawo koyatsidwa kumakhala kofooka kwambiri, ndipo coagulant iyenera kuwonjezeredwa kuti ifupikitse nthawi ya coagulation.The plasma separation gel anticoagulation chubu amapopera mankhwala anticoagulants monga lithiamu heparin pa khoma lamkati la kupatukana gel osakaniza magazi chubu kuti akwaniritse zosowa mofulumira plasma biochemical kuyezetsa mwadzidzidzi.

Mu ntchito zothandiza, nthawi zambiri amakumana kuti kulekana kwa kupatukana kwa gel osakaniza magazi chubu si zabwino, mwachitsanzo: ena kupatukana machubu mphira, zikhoza kuoneka kuti kupatukana zidutswa za gel osakaniza kapena madontho a mafuta akuyandama pamwamba. seramu kapena kuyimitsidwa mu seramu;gel olekanitsa wosanjikiza amayandama pa seramu wosanjikiza.pamwamba ndi zina. Kulekanitsa ma gel osakaniza kungathenso kusokoneza zotsatira za mayeso.Mu dipatimenti yathu, zapezeka kuti gulu lenileni la reagents ndi seramu kupatukana gel osakaniza imathandizira chubu anachita ndi wina ndi mzake pa HBSAg kudziwika dongosolo Abbott i2000SR kudziwika, kumabweretsa zotsatira zabodza.

Pepalali makamaka limasanthula kuchokera ku mbali ziwiri, ndiko kuti, zifukwa za kulephera kulekanitsa gel olekanitsa, ndi chikoka cha kuyambitsa gel olekanitsa pa muyeso.

1. Njira yolekanitsira seramu ndi madzi a m'magazi mwa kulekanitsa gel osakaniza gel osakaniza ndi thixotropic mucocolloid wopangidwa ndi hydrophobic organic mankhwala ndi silika ufa.Kapangidwe kameneka kali ndi ma hydrogen bond ambiri.Kukhalapo kwa ma hydrogen bonds kumapanga maziko a mankhwala a thixotropy a gel olekanitsa..Kukoka kwapadera kwa gel olekanitsa kumasungidwa pa 1.05, mphamvu yokoka ya chigawo chamadzimadzi chamagazi ndi pafupifupi 1.02, ndipo mphamvu yokoka ya gawo lopangidwa ndi magazi ndi pafupifupi 1.08.Pamene gel olekanitsa ndi magazi coagulated (kapena anticoagulated magazi athunthu) ndi centrifuged mu chiyeso chomwecho chubu, Chifukwa cha mphamvu centrifugal ntchito pa olekanitsa gel osakaniza, hydrogen chomangira maukonde dongosolo wathyoledwa mu unyolo ngati dongosolo, ndi kulekanitsa. gel osakaniza amakhala otsika mamasukidwe akayendedwe madzimadzi.Chifukwa cha mphamvu yokoka yosiyana, gel olekanitsa amasinthidwa ndikusinthidwa kuti apange zigawo zitatu za magazi (anticoagulated magazi athunthu) / kulekanitsa gel / seramu (plasma).Pamene centrifuge imasiya kuzungulira ndikutaya mphamvu ya centrifugal, tinthu tating'onoting'ono ta gel olekanitsa timapanganso maukonde ndi zomangira za haidrojeni, kubwezeretsanso gawo loyamba la gel osakaniza, ndikupanga kusanjikiza pakati pa seramu (plasma) ndi magazi (anticoagulated). magazi onse)..

2. Zifukwa zoipa kulekana zotsatira kulekanitsa gel osakaniza

2.1 Kulekanitsa Ubwino wa Gel Mphamvu yokoka ya gel yolekanitsa ili pakati pa seramu (plasma) ndi maselo a magazi, omwe ndi maziko a thupi la kusinthika kwa gel olekanitsa ndi kulekanitsa kwa seramu (plasma).Ngati khalidwe la gel olekanitsa la chubu chosonkhanitsira magazi ndi losauka ndipo mphamvu yokoka yeniyeni sikugwirizana ndi zofunikira, izo zidzakhudza kwambiri zotsatira za kulekanitsa seramu (plasma), ndi chodabwitsa kuti gel olekanitsa ndi seramu (plasma) ndi. zolumikizana zitha kuchitika.

2.2 Kutsekeka kosakwanira kwa magazi Pambuyo pa centrifugation, nthawi zina chipinda cha gel olekanitsa ndi seramu ndi magazi a magazi sizimalekanitsidwa kwathunthu, ndipo fibrin filaments imapezeka mu seramu.Chifukwa chake nthawi zambiri ndikuti magazi samatsekeka kwathunthu pamaso pa centrifugation.Kuchulukana kwa magazi kosakwanira kungapangitse fibrin kusakanizidwa mumkhalidwe wodzipatula.Chubu cha mphira cholekanitsa seramu chiyenera kugwiritsidwa ntchito moyenera molingana ndi malangizo, ndipo seramu ikhoza kukonzedwa ndi centrifugation magazi atatha kukhazikika (nthawi zambiri, chubu la pulasitiki lomwe lili ndi coagulant liyenera kuyikidwa mowongoka kwa mphindi 30, ndi magazi chubu chosonkhanitsira popanda coagulant chiyenera kuyikidwa mowongoka kwa mphindi 60-90).zitsanzo zapamwamba za seramu.

2.3 Kutentha kwa centrifugation Kutentha kwa centrifugation kumakhudza kwambiri zotsatira za kulekanitsa seramu ndi chubu la gel olekanitsa.Seramuyo inali yomveka bwino mu chubu cholekanitsa cha gel osungunula, chosiyanitsidwa ndi centrifuge wamba pa kutentha kwa chipinda, koma mikanda yamafuta yamitundu yosiyanasiyana idawoneka mu 15% mpaka 20% ya zitsanzo.Kumbali inayi, palibe mikanda yamafuta yomwe idapezeka mu seramu yolekanitsidwa ndi chubu choyesera chokhazikika ndi centrifuge yotsika kutentha.Pamene kutentha kupitirira kutentha kosungirako komwe kumafunika kuti gel olekanitsa, gel osakaniza asungunuke mu seramu.Sizingotsekereza ndikuyipitsa singano yachitsanzo ndi kapu yochitira za biochemical analyzer, komanso zimakhudza kwambiri zotsatira za kuyeza kwazachilengedwe.