PRP Tubes Acd Tubes
Kufotokozera Kwachidule:
Anticoagulant Citrate Dextrose Solution, yomwe imadziwika kuti ACD-A kapena Solution A ndi njira yopanda pyrogenic, yosabala.Izi zimagwiritsidwa ntchito ngati anticoagulant popanga plasma-rich plasma (PRP) yokhala ndi PRP Systems pokonza magazi a extracorporeal.
Anticoagulant Citrate Dextrose Solution, yomwe imadziwika kuti ACD-A kapena Solution A ndi njira yopanda pyrogenic, yosabala.Izi zimagwiritsidwa ntchito ngati anticoagulant popanga plasma-rich plasma (PRP) yokhala ndi PRP Systems pokonza magazi a extracorporeal.Ma anticoagulants omwe ali ndi citrate amagwiritsa ntchito mphamvu ya citrate ion ku chelate calcium ionized calcium yomwe imapezeka m'magazi kuti iteteze kusungunuka kwa magazi ndikupanga chitsulo chosapanga ionized calcium-citrate.
Mankhwala okhawo a anticoagulant omwe bungwe la United States Food & Drug Administration lavomereza kuti ligwiritsidwe ntchito pokonzekera PRP mu PRP Systems zosiyanasiyana ndi ACD-A.Malinga ndi kafukufuku yemwe adachitika mu 2016 pa PRP yomwe idapezedwa ndi ma anticoagulants osiyanasiyana komanso zotsatira zake pamakhalidwe a ma cell a mesenchymal stromal mu vitro ndi manambala apulateleti, pali zotsatira zabwino pakugwiritsa ntchito PRP kukonza minofu ya minofu.
Popatula mapulateleti amalangizidwa kuti asinthe sodium citrate ya Acid Citrate Dextrose (ACD-A) chifukwa njira yodzipatula imafuna masitepe angapo ochapira.Mapulateleti amakhala okhazikika pa 37C panthawi yopota, koma kuwazungulira kutentha (25 C) kumagwiranso ntchito bwino.Kuchepetsa pH kudzera mu ACD-A (ikufika pafupi ndi 6.5) kumathandizira kusokoneza machubu otsalira a thrombin m'machubu apulateleti, ndipo kumathandizira kukonzanso kachitidwe ka zinthu za m'mwazi pamene kusandutsa kugwira ntchito kwa minima.Nthawi zambiri muyenera kuyimitsanso mapulateleti ku Tyrode Buffer yoyenera (pH 7.4) kuti mubwezeretse magwiridwe antchito awo.ACD ili ndi zabwino zambiri pankhani yosunga mapulateleti
Pamene ACD idagwiritsidwa ntchito, zotsatira zake zidawonetsa zokolola zapamwamba zamagazi m'magazi onse.Komabe, kugwiritsiridwa ntchito kwa EDTA kunalimbikitsanso kukula kwa voliyumu ya platelet pambuyo poti ma centrifugation a magazi apangidwa kuti apeze PRP.Kenako, kugwiritsa ntchito ACD kunapangitsa kuti ma cell a mesenchymal stromal achuluke.Chifukwa chake, zidatsimikiziridwa kuti anticoagulants kuphatikiza ACD-A amagwira ntchito yayikulu pakukonzekera kwa PRP ndikuthandizira kukhathamiritsa kwambiri ntchitoyi.
